We determined the effect of moderate intensity physical activity on glycemic control and antioxidant status in prediabetic population. This experimental study was carried out in Physiology department, Institute of Basic Medical Sciences, Khyber Medical University, Peshawar, Pakistan. A total of 50 adult prediabetic subjects having 22 females and 28 males with the age range of 18 to 35 years were included. Diagnosis of prediabetes was made by glycated hemoglobin falling in the range of 5.7-6.4%, and impaired fasting glucose (100-125 mg/dL). Anthropometric measurements and biochemical assays were carried out at preand post-exercise intervention. The participants performed moderate exercise of 30 min with heart rate max 70% ± 5% for 5 days a week for 8 weeks, monitored with pedometer. Enzyme-linked immunosorbent assay was carried out for individual and total antioxidants. Anthropometric parameters showed a significant decrease at post exercise analysis. Similar changes were observed for fasting glucose (p<0.001) and glycated hemoglobin (p<0.001). Slight increase in uric acid (p<0.005) and total antioxidant concentration (p<0.001) were found. However, superoxide dismutase, glutathione peroxidase, vitamin C, and nitric oxide decreased (p<0.001). Moderate physical activity for 8 weeks significantly reduced the individual antioxidant levels, nominal increase in total antioxidant capacity and uric acid, and there was an explicit decline in the anthropometric and diabetic profile of prediabetic population.