
Samreen Riaz
University of the Punjab, PakistanTitle: Evaluation of Arsenic as Diabetogenic Agent in Type 2 Diabetes Mellitus and its Molecular Basis
Abstract
Millions of people
die each year due to diabetes, it occurs in all groups of ages, but there is no
proper cure for this disease. There are so many causes of diabetes like poor
lifestyle, genetic factor, and environmental pollutants. Arsenic and its
compounds are involving in the damaging of beta cells by different mechanisms
that includes oxidative stress, production of free radicals and disturbance of
protein hormone which ultimately destroy insulin sensitivity. In this research
I have studied the association between type 2 diabetes and arsenic related
compounds. The first step in the research is to collect the sample of 1000
people in which 500 is control and 500 is diabetic patient. Then physical such
as height, weight, daily activates was determined and biochemical parameter was
determined which are Bilirubin, Urea, HBA1c AST, ALP and Creatinine.The total
serum protein in samples was determined by performing Bradford Protein
Estimation Assay. The control and diabetic patient’s protein concentration was compared. The serum protein analysis was done by SDS
PAGE. SDS PAGE is a technique used for visualization and separation of proteins
on the basis of difference in their molecular masses and size. The samples were
prepared and run through the gel and the results were analyzed. Then Arsenic
was determined. Glucose level and all other parameter was higher in diabetic
patients was determined through statistically between normal and diabetic
patient. So it is proved that Arsenic is responsible to cause diabetes. So in
order to reduce diabetic patient we should adopt life style modification in
which proper diet, exercise is included. We increase the usage of “personal
protective equipment” to avoid arsenic exposure.
Biography
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