
Dinithi Peiris
University of Sri Jayewardenepura, Sri LankaTitle: A novel compound isolated from Passiflora suberosa with potential antidiabetic activity
Abstract
Passiflora suberosa L. belongs to the family
Passifloraceae and is commonly known as "wild
passion fruit"/"Thiththa wel" is used in traditional
medicine against diabetes. To date, hypoglycemic
potential of P. sbersoa has not been
reported nationally and internationally. The
present study focused on biologically guided
fractions for P.subersoa to identify inhibitory
activities of carbohydrate hydrolysis enzymes and
isolate a novel therapeutic active molecule against
hypoglycemia. The methanol extract
of P.subersoa was partitioned with hexane,
chloroform, and ethyl acetate, and Invitro hypoglycemic activity was evaluated using ?amylase and ?-glucosidase inhibitory activities.
The ?-amylase inhibitory and ?-glucosidase
inhibitory activities were determined. The ethyl
acetate fraction was selected for compound
isolation using silica gel 60 and Sephadex LH20
column chromatography through bioactivity
guided fractionation. Once an active sub-fraction
of chromatography was established, it was further
purified using reverse-phase HPLC
purification and liquid chromatography-mass
spectrometry (LC-MS) analysis. Finally, the
structural elucidation was carried out using nuclear
magnetic resonance (NMR) analysis. The ethyl
acetate fraction exhibited the highest ?-amylase
(IC50: 284.63±0.51 ?g/mL) and ?-glucosidase
(IC50: 120.80±1.33 ?g/mL) inhibitory activity
compared with the positive control (IC50?-amylase:
197.51±0.32 ?g/mL; IC50?-glucosidase 116.62±1.41
?g/mL). The structure was elucidated using NMR
spectroscopy after purification of active subfraction by reverse-phase HPLC and liquid
chromatography-mass spectrometry (LC-MS). The
structural elucidation data suggested a novel
organic compound “A” was isolated. According to
the results, alpha-amylase inhibitory activity was
exhibited by the isolated compound was (IC50:
133.51?g/mL) compared to the positive control
(IC50 =197.51±0.32 ?g/mL). Similar inhibitory
activity was observed in an alpha-glucosidase
inhibitory activity where of the isolated compound
exhibited (IC50: 154.39 ?g/mL) compared with the
positive control (IC50 =116.62±1.41 ?g/mL).
Biography
Dinithi Peiris has completed his PHD in 1998 from
the University of Sheffield, UK. She currently
serves as a professor at University of Sri
Jayewardenepura, Sri Lanka She has supervised
many postgraduate and undergraduate students and
authored numerous publications that have been
cited over 500 times, and his publication h-index is
15.